- The Protagonist (主人公 Shujinkou?, translated as "You" on the card) is the main character of Digimon World. He was brought to the Digital World by Jijimon to save File Island from being destroyed by evil Digimon, who were losing their memory and turning evil. He can start with a number of Digimon, but the ending shows that he has a Mamemon, who calls him back to defeat a revived Analogman.
- Analogman is the main human villain and a hacker of great skill. He believes that Digimon should all be slaves to humankind. His Digimon partner is Machinedramon.
- The Protagonist's mother.
- Akira (アキラ?) is the main character of Digimon World 2. He had many different partners over the course of the game but he started out with the partners Patamon, Agumon, or DemiDevimon (which is canon to the story, but in-game Akira has the choice of becoming a Black Sword, Gold Hawk, or Blue Falcon Tamer). Later on, he also befriends a MetalGreymon in the BIOS Domain. His Digi-Beetle is named Gunner (ゼロガンナー Zero Gan'nā?, lit. "Zero Gunner").
- OverLord GAIA (オーバーロードＧＡＩＡ Ōbārōdo GAIA?) is the main antagonist of Digimon World 2. He is actually the mother computer used in a project called "GAIA", which happened in the real world. Despite being just a machine, GAIA has the ability to think and act on his own.
- Mission Chief Carol (カリル管理官 Kariru-kanrikan?, lit. "Commissioner Khalil")
- Bertran (ベルトラン Berutoran?) is Akira's chief rival and always attempts to outdo and surpass Akira with little success. He is partnered with a Digitamamon, an Okuwamon, a Ninjamon, a Tankmon, and a Deramon.
- Joy-Joy (リンリンシャオ Rin Rin Shao?, lit. "Lin Lin Shao") is Bertran's best friend and is always around him, trying to calm him down when he gets frustrated with Akira. Despite her friendship to Bertain, she seems to be fairly kind with Akira, although she does get mad at him when he defeats her Digimon in the Colosseum. She is partnered with a Monzaemon, a Deramon, a Starmon, a Ninjamon, and a Raremon.
- Kim (アユミ Ayumi?) is a Tamer who infiltrates the city and steals the Generator Parts from the Tamer's Club. She is thought to be a villain, but after Akira defeats her Digimon in Modem Domain he discovers that she was only borrowing the Generator Parts for her sister to invent something new for the Digital World. In the ending, it seems she becomes attracted to Akira. She is partnered with a Wizardmon, a Seadramon, an Akatorimon, and an Ogremon.
- Ben Oldman is the former leader of the Blue Falcons, went into hiding after the "Blood Knights Incident". He held the secret to reaching File Island. He is partnered with a Centarumon, a MetalMamemon, and a Meramon.
- Techna-Donna (ドランドナ Dorandona?) is Kim's sister, who gives Akira the Power Radar after he realizes that the standard radar isn't powerful enough for certain domains, specifically the Giga Domain.
- Professor Piyotte (ヒョット博士 Hyotto-hakase?, lit. "Professor Hyotto") is an expert in Digimon DNA and knows a great deal about Digimon and DNA digivolving. Akira comes to the professor to ask him where the Archive Ship is. He is partnered with a Yanmamon, a Syakomon, and an Ikkakumon.
The Blood Knights (ブラッドナイツ Buraddo Naitsu?) are a group of Guard Tamers who rebelled and started a war.
- Crimson (クリムゾン Kurimuzon?) is the leader of the Blood Knights. He eventually merges with ChaosBlackWarGreymon, ChaosMetalSeadramon, ChaosPiedmon, and Chaos Lord to become NeoCrimson, a powerful but uncontrollable Digimon who is killed by Akira. He is partnered with a Deltamon, an ExTyrannomon, a Myotismon, a Pumpkinmon, a SkullMammothmon, and a VenomMyotismon.
- Damien (ダメダボース Damedabōsu?) is a commander in the Blood Knights. He is often boastful but is constantly beaten by Akira. He tries to infiltrate the city by impersonating a Black Swords Tamer. He is partnered with a Cherrymon, an Etemon, a MegaKabuterimon (Blue), an Ogremon, a Puppetmon, and a RedVegiemon.
- Vandar (バンダール Bandāru?) is the leader of the Gold Hawks. He is partnered with a MetalMamemon, a Mamemon, and a PrinceMamemon.
- Esmeralda (エスメラルダ Esumeraruda?, Esp: Emerald) is a Gold Hawk Tamer who isn't very confident. Brian tries to comfort her, and she is also Akira's companion throughout the game. She is partnered with a Tentomon, a Biyomon, a Tapirmon, a Piddomon, an Unimon, and a Birdramon.
- Mark Shultz (アークシュルツ Āku Shurutsu?, lit. "Ark Shultz") is a Gold Hawk Tamer who tells Akira about a MetalGreymon in the BIOS Domain. Akira eventually befriends this MetalGreymon using the Toy Plane he gives Akira. He is partnered with a Saberdramon, a Gururumon, and a ShimaUnimon.
- Brian Wiseman (ブランワイスマン Buran Waisuman?, lit. "Bran Wiseman") is a high ranking Gold Hawk Tamer who seems to have a romantic interest in Esmeralda. He battles Akira in the Colosseum. He is partnered with a Garudamon, an AeroVeedramon, and a Mammothmon.
- Cecilia (セシリア Seshiria?) is the leader of the Blue Falcons and also the head trainer for students becoming Digimon Tamers. Akira joins her team after his last mission in becoming a Tamer is complete (canon to the story, but in-game Akira has the choice of joining the Black Swords or Gold Hawks). She is partnered with a Jijimon, a Magnadramon, and a MarineAngemon.
- Doug Duem (ヤルヤル Yaruyaru?) is a Blue Falcon Tamer who tries to be very aggressive but fails. He is partnered with a Elecmon, a Gotsumon, a Floramon, a Tyrannomon, and a Centarumon, Monochromon.
- Debbie (ヴィーヴィ Vīvi?, lit. "Vivi") is Blue Falcon Tamer who usually hangs around in the Tamer's Club, where she has her dancing Palmon that a Black Swords Tamer eventually falls in love with when Debbie leaves. She is partnered with a Palmon, a MoriShellmon, a JungleMojyamon, a Togemon, and three Mamemon.
- Zudokorn (ズドカーン Zudokān?) is a member of the Blue Falcons Guard Team and also someone that Akira looks up to. He helped Akira with his last mission to get his Taming License. He is partnered with a MegaSeadramon named Akagi (シドラン Shidoran?, lit. "Seadran"), a Lillymon named Naomi (リリーナ Rirīna?, lit. "Lilyna"), a Triceramon named Dinogon (トリケラス Torikerasu?, lit. "Triceras"), a Blossomon, a Deramon, and a Pumpkinmon. He lends the first three to Akira and uses the last three for Colosseum battles.
- Skull (スカール Sukāru?) is the leader of the Black Swords. He is partnered with a Hagurumon, a Puppetmon, and a DemiDevimon.
- Sheena (オトタテシナ Ototateshina?) is a Black Sword Tamer who isn't very confident and also informs Akira that Agumon was looking for him in the Digimon Trading Center. She is partnered with an Otamamon, a DemiDevimon, a Gazimon, a Gesomon, a Devimon, and a Guardromon.
- Chris Connor (クロンガンダー Kuron Gandā?, lit. "Chrome Gander") is a Black Sword Tamer who gives Akira a Toy Plane in the Digimon Trading Center. Later on, he goes to File Island and becomes confused by Gekomon in the Data Domain and mistakes Akira for an enemy. His partners are a Bakemon, Woodmon, Soulmon, Phantomon, Myotismon, Megadramon.
- Karen Bates (キルリベイト Kiruribeito?) is a high ranking Black Sword Tamer, who first appears at Angemon's house. Her partners are a Megadramon, a WaruMonzaemon, and a Tekkamon.
- Junior (アツシ Atsushi?) is the main character of Digimon World 3.
- Teddy (タクミ Takumi?) is one of Junior's friends and apparently idolizes a famous hacker known as Lucky Mouse.
- Ivy (ミノリ Minori?) is one of Junior's friends. Her Digimon Online alias is Kail (カイト Kaito?, lit. "Kite"). According to Junior, she hadn't shown any interest in Digimon before. Later, it turns out she wanted to find her brother, Kurt.
- Kurt is a character whose Digimon Online alias is Lucky Mouse. He helps out Junior in some situations, disguised as a Numemon. He is later revealed to be Ivy/Kail's brother, shortly before he is turned into an Oinkmon by the A.o.A. In the PAL version of the game, Kurt is revealed to be the new MAGAMI president and World Champion. He is partnered to a Datamon, a Bulbmon, and an Armageddemon.
International Police S.A.PEdit
International Police S.A.P is an international police agency who fight against terrorism.
- Lisa (リサ Risa?) is is the leader of the group that entered Digimon Online to investigate the A.o.A.
- Nick (ニック Nikku?) is a member.
- Keith (キース Kīsu?) is a member and partnered to a Triceramon, a Kuwagamon, and a Kunemon.
- Account Clerk (アカウントがかり Akaunto Gakari?) is a clerk in the MAGAMI Online Center.
- Operator (オペレーター Operētā?) is the responsible for the Chamber Room.
- Account Clerk (あんないがかり An'nai Gakari?, lit. "Usher") is the person who welcomes new players in the Login Room.
- Tamer Service (テイマー・サービス Teimā Sābisu?) is an attendant in the Main Lobby of Asuka City. She downloads the data of new players' Digimon Partners to their Satellites.
- Game Master (ゲームマスター Gēmu Mastā?) is partnered to a Minotarumon, a Gargoylemon, and a Persiamon.
- Seiryu Leader (セイリュウ・リーダー Seiryū Rīdā?) is partnered to a Minotarumon, a Tyrannomon, and an Apemon.
- Suzaku Leader (スザク・リーダー Suzaku Rīdā?) is partnered to a Woodmon, a RedVegiemon, and a ShogunGekomon.
- Fake Byakko Leader is partnered to two Numemon.
- Byakko Leader (ビャッコ・リーダー Byakko Rīdā?) is partnered to a Flymon, a Tortomon, and a Mamemon.
- Genbu Leader (ゲンブ・リーダー Genbu Rīdā?) is partnered to a Vikemon, a MetalEtemon, and an Antylamon.
- Ice Witch is partnered to a Frigimon, a MegaSeadramon, and a Mammothmon.
- Fire Knight is partnered to a Meramon, a SkullMeramon, and a BlueMeramon.
- Dark Knight is partnered to a SkullSatamon, a Devidramon, and a Vilemon.
- Tamer Genji (#1) (テイマー・ゲンじい Teimā Genjii?, lit. "Tamer Genjii") is partnered to a Kuwagamon. He is the first player Junior meets in the Digital World. When Junior exits goes to Asuka City for the first time, Tamer Genji asks if he's a new a player and decides to battle him, telling the boy to not worry if he doesn't understand the rules and that gaining experience is the important thing. After Junior defeats him, Genji tells him that he has potential to become "World Champ", and tells him to go to the Digimon Lab and learn more about Digimon before leaving the city. He also tells him to go to Yellow Cruiser to learn about card Battles.
- Tamer Genji (#2) is partnered to two Kuwagamon.
- Tamer Natsumi is partnered to a Betamon, a Vegiemon, and a Kiwimon.
- Tamer Akiba is partnered to a Tapirmon.
- Tamer Chris is partnered to a Kuwagamon, a Flymon, and a Yanmamon.
- Tamer Tomomi is partnered to a Betamon, a Kiwimon, and a Vegiemon.
- Tamer Andy is partnered to a Triceramon and a Tuskmon.
- Tamer George is partnered to a Vademon and a Thundermon.
- Tamer Mei Lin is partnered to a DemiDevimon and a Bakemon.
- Tamer Catherine is partnered to two Kunemon and a Dokugumon.
- Tamer Lucia is partnered to a Goblimon and a Ogremon.
- Tamer Robert is partnered to a Crabmon, a Gizamon, and a Gekomon.
- Tamer Gordon is partnered to a Crabmon, a Gizamon, and a Gekomon.
- Tamer Alice is partnered to a Kunemon and two Dokugumon.
- Tamer Mitch is partnered to a Yanmamon, a Kuwagamon, and a Flymon.
- Tamer Bob is partnered to a Coelamon, a Kokatorimon, and an Airdramon.
- Tamer Jessica is partnered to a Seadramon, a Kokatorimon, and a Shellmon.
- Tamer Nakano is partnered to a Tapirmon.
- Tamer Makoto is partnered to a Brachiomon, a Deltamon, and a MetalTyrannomon.
- Tamer Brown is partnered to a Brachiomon, a Deltamon, and a MetalTyrannomon.
- Tamer Haruka is partnered to a Vilemon, a Phantomon, and a Mummymon.
- Tamer Poemy is partnered to a Dolphmon, a Monzaemon, and a ShogunGekomon.
- Tamer Pierre is partnered to a Crabmon, a Gizamon, and a Gekomon.
- Tamer Shingo is partnered to two Kuwagamon, and an Okuwamon.
- DRI Jimmy—Partners: Guilmon.
- DRI Johann—Partners: Agumon.
- DRI Kazuki—Partners: Patamon.
- DRI Michael—Partners: Renamon.
- DRI Ken—Partners: Kotemon.
- DRI Yuji—Partners: Bearmon.
- DRI Yang—Partners: Monmon.
- DRI Bob—Partners: Veemon.
Real World (Undersea Base Magasta)Edit
- Mech Soldier is partnered to a Dragomon, a Pukumon and a Giromon.
- Royal Guard is partnered to a Whamon, a Vademon, and a Machinedramon.
Real World (Gunslinger Satellite)Edit
- Metal Soldier is partnered to a Infermon, and an Apocalymon.
- Lord Megadeath is partnered to a Fuujinmon, a Suijinmon, and a Raijinmon.
- MAGAMI President is partnered to a MetalSeadramon, a VenomMyotismon, and a Raidenmon.
- Qing Long Chief (チンロン・チーフ Chinron Chīfu?) is partnered to a Deltamon, a Triceramon, and a Kimeramon.
- Zhu Que Chief (スーツェイ・チーフ Sūtsei Chīfu?) is partnered to a Cherrymon, a Scorpiomon, and a Snimon.
- Bai Hu Chief (バイフー・チーフ Baifū Chīfu?) is partnered to a Golemon, a Gryphonmon, and a Ebidramon.
- Xuan Wu Chief (シェンウー・チーフ Shen'Ū Chīfu?) is partnered to a Boltmon, a SkullMammothmon, and a Puppetmon.
- Ice Master is partnered to a Vikemon, an Antylamon, and a Scorpiomon.
- Fire Master is partnered to two Lynxmon, and a Creepymon.
- Dark Master is partnered to a Ghoulmon, a Piedmon, and an Infermon.
- Tamer Heinrich is partnered to a Vademon, a Thundermon, and a Pukumon.
- Tamer Murdock is partnered to a MarineDevimon, and a Dragomon.
- Tamer Takuya is partnered to a Kuwagamon, an Okuwamon, and a HerculesKabuterimon.
- Guard Banch is partnered to a Cyclonemon, and a Megadramon.
- Tamer Mai is partnered to a Hagurumon, a Clockmon, and a KingEtemon.
- Tamer Gon is partnered to a Kokatorimon, a Cherrymon, and an Ebidramon.
- Veemon, Guilmon, Agumon, Dorumon: The main protagonists.
- Leomon: A digimon commander of DSG who goes to investigate the mysterious Death Valley but is captured by evil digimon, later he is found in the Mecha Rogue X lab where his power is drained to create Mecha Rogue X1, he is saved but loses his memory of the operation.
- Ophanimon: One of the digimon chiefs of DSG who maintains order in the Digital World. Ophanimon is a male in this game.
- Seraphimon: Ophanimon's partner and a digimon chief of the DSG, he is reawakened by going into the freezer of DSG and turning on the computer. Seraphimon quickly fixes the system. Seraphimon is believed to be a digimon of great power. He is wise and apparently knows a lot about Mecha Rogue X.
- Mecha Rogue X: An experiment thought to be created by Yggdrasill.
- The protagonist in this game is a new Card Tamer entering the Digital World. The protagonist is dressed similarly to Hiro from Digimon World, but his appearance is only a virtual avatar of the true protagonist; his name defaults to just "PLAYER".